IN SITU HYBRIDIZATION

in situ hybridization: translation

(1) Technique for revealing patterns of gene expression in a tissue. The tissue is fixed and prepared (usually by sectioning) on a slide, and a labelled DNA or RNA probe is hybridized to the sample. It binds only to complementary mRNA sequences, and so only stains cells that are expressing the gene in question.

(2) Use of the technique for identifying the position of a gene on a chromosome by hybridizing a probe to spread chromosomes. Widely used in Drosophila salivary gland polytene chromosome "squashes", but now also possible with a variety of eukaryotic nuclei. ‘FISH’ analysis is the use of in situ hybridization.

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IN SITU HYBRIDIZATION

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